
In conclusion, exogenous calcium could alleviate the inhibition caused by acid rain on soybean growth by increasing the activity of plasma membrane H +-ATPase for providing driving force to nutrient absorption, and its regulating effect was limited by intensity of acid rain. Higher activity of plasma membrane H +-ATPase in soybean treated with acid rain at pH 3.0 and exogenous calcium was good to uptake of nutrients and promoted the recovery of soybean growth, compared with soybean treated with acid rain at pH 3.0.

After a 5-day recovery (without acid rain stress), all parameters in soybean treated with acid rain at pH 3.0 and exogenous calcium were still worse than those of the control, but obviously better than those treated with acid rain at pH 3.0. In addition, exogenous calcium (5 mM) alleviated the inhibition induced by pH 3.0 acid rain on the activity of plasma membranes H +-ATPase and the expression of GmPHA1 at transcriptional level, being benefiting to maintaining uptake of nutrients (NO 3 -, P, K, Mg, and Zn), and then lower the decrease in dry weight biomass and chlorophyll content. However, all parameters in soybean treated with exogenous calcium (5 mM) and acid rain at pH 4.5 were closed to the control levels. Our results show that acid rain (pH 4.5/pH 3.0) caused decreases in dry weight biomass, chlorophyll content and uptake of nutrients elements (NO 3 -, P, K, Mg, Zn and Mo) and an increase in membrane permeability of root. Here, we studied whether calcium played a role in enhancing tolerance of plants to acid rain stress by hydroponics and simulating acid rain stress.

Calcium (Ca) is one of essential elements for plant growth and development, and also plays a role in regulating plant cell physiology and cellular response to the environment.
